Tel: +86-021-3762 1270
Fax: +86-021-6762 1877
Email: service@genicbio.com
GenicBio offers a full range of services for both polyclonal and monoclonal antibody development and production. Our custom antibodies can be generated against customer-supplied antigens or against peptides synthesized and conjugated by us. Our scientists have the knowledge and experience in antigen design, antibody production, purification and isolation, etc., which allows us to produce high-affinity, titer guaranteed and customer-satisfied antibodies.
| Cat.No. |
Polyclonal Antibody Production Packages |
Guarantees and Deliverables |
|
GPAB01 |
Anti-protein pAb package: $420.00 Antigen: Protein, 3-5mg with >85% (provided by customer) Animal: 2 rabbits or 5 mice Delivery time:: 9~10 weeks Service Contents: Immunization & Anti-Sera Production, ELISA identification |
Guarantees: ELISA >1:200,000 Deliverables: Pre-immune serum: 2ml/rabbit Original sera volume: 40~60ml from each rabbit Titer (ELISA) result and product report |
|
GPAB02 |
Customer supplied antigen standard package: $500.00 Antigen: Peptide, 5-10mg with >80% (provided by customer) Animal: 2 rabbits or 5 mice Delivery time:: 9~10 weeks Service Contents: Conjugation, Immunization & Anti-Sera Production, ELISA identification |
Guarantees: ELISA >1:100,000 Deliverables: Pre-immune serum: 2ml/rabbit Original sera volume: 40~60ml from each rabbit Titer (ELISA) result and product report |
|
GPAB03 |
Complete anti-peptide pAb package: $600.00 Antigen: Peptide, GenicBio to synthesize (up to 15aa, 20mg, >75% purity) Animal: 2 rabbits or 5 mice Delivery time:: 11~12 weeks Service Contents: Peptide design & peptide synthesis, conjugation, Immunization & Anti-Sera Production, ELISA identification |
Guarantees: ELISA >1:100,000 Deliverables: 10mg unconjugated peptide Pre-immune serum: 2ml/rabbit Original sera volume: 40~60ml from each rabbit Titer (ELISA) result and product report |
|
GPAB04 |
Phospho-specific pAb package: USD1,500.00 Antigen: Phospho peptide, GenicBio to synthesize(purity >90%) Animal: 2 rabbits or 5 mice Delivery time:: 12~14 weeks Service Contents: Peptide design & peptide synthesis, conjugation, Immunization & Anti-Sera Production, antigen affinity purification, ELISA identification |
Guarantees: ELISA >1:100,000, 10 × greater than non-phosphorylated peptide Deliverables: 10mg of phospho & non-phospho peptides Pre-immune serum: 2ml/rabbit Affinity purified anti-phospho-peptide specific antibody that only reacts with phosphorylated site |
|
GPAB05 |
Site specific pAb package - email us for a quote Antigen: methylated or acetylated peptide, GenicBio to synthesize(purity >90%) Animal: 2 rabbits or 5 mice Delivery time:: 12~14 weeks Service Contents: Peptide design & peptide synthesis, conjugation, Immunization & Anti-Sera Production, antigen affinity purification, ELISA identification |
Guarantees: ELISA >1:100,000, 10 × greater than non-methylated(or acetylated) peptide Deliverables: 10mg of methylated & non-methylated peptides Pre-immune serum: 2ml/rabbit Affinity purified anti-phospho-peptide specific antibody that only reacts with methylated site |
Optional:
• Protein A/G affinity purification: USD130.00
• Peptide affinity purification: USD280.00
• Animal maintenance: USD1.00 per animal per day. Extra bleed: $20.00 per collection per animal.
Monoclonal Antibody Services
• Antigens (recombinant proteins or peptides) provided by customers or produced at GenicBio
• Various packages to meet different needs
• Free antigen design
• ELISA titers are guaranteed to reach >10,000
• Phosphor-specific and other modification-specific antibodies
GMAB01: Anti-protein mAb package - $2,700.00(4~5 months)
Antigen: purified protein, at least 3mg with >90% (provided by customer, lyophilized powder).The development protocol is same as GMAB02 (without peptide synthesis and conjugation)
GMAB02: Complete anti-peptide mAb package - $2,950.00(5~6 months)
1. Peptide Synthesis and Conjugation
- Peptide design: Free of charge
- Peptide synthesis: (up to 15aa, 20mg and purity>90%); A Cys will be added to either end of the peptide for conjugation
- Conjugation: KLH, BSA, OVA or others
2. Immunization and ELISA Screening
- The conjugated peptide is used to immunize five Balb/c mice (4-5 injections)
- Tail bleeds are screened by ELISA (WB、IHC、IF and other screening methods are optional)
3. Fusion and screening
- Perform splenocyte fusion on the mice that shows best response to the antigen
- The hybridoma cells are expanded and screened by ELISA (WB、IHC、IF and other screening methods are optional)
4. Subcloning and Ascites Production
- Subclone two (up to five) positive parental clones by limiting dilution and isotyped.
- Two positive clones are Cryopreserved and used to produce ascites.
5. Delivery and report
- 10mg un-conjugated peptide
- 1 vial of cell culture for each hybridomas cells
- 5 ml of ascites produced from each of the hybridomas cells with ELISA titer> 1:5,000
GMAB03: Phospho-specific mAb package(5~6 months)
Please email us your requirement for a detail quote.
